Methods used in the Fangman/Brewer lab

and other stuff

Contents

Methods

DNA preps

2-D gels

Replication timing

Transformation

Microarray paper (Raghuraman, Winzeler, Collingwood et al., Science, 2001)

Abstract

Full Text

Supplemental Data

Raw data files

%HL data (unsmoothed)

%HL data (smoothed)

Smoothed replication profiles (trep values vs. chromosome coordinates)

Origin predictions

Replication profiles (2.5 MB pdf file)

 

Composition

Methods

DNA Preps

Yeast "smash-&-grab" DNA prep

Yeast genomic DNA prep for 2-D gels

Preparation of yeast DNA embedded in agarose plugs

Gel-purified, agarased YACs for yeast transformation

2-D Gel Electrophoresis

Standard 2-D gels

Fork-direction gels

Replication Timing

Density transfer

Comparative hybridization

Transient hemimethylation

Transformation

Yeast transformation using frozen competent cells

Gel-purified, agarased YACs for yeast transformation

Flow Cytometry information

Introduction to Flow Cytometry--UMass Amherst

Univ. of Florida Flow Cytometry pages

Mostly yeast

Yeast labs--Home pages and Research Summaries

Bonny Brewer's home page

Walt Fangman's home page

 

David Botstein's lab

Jim Broach's lab

Trisha Davis' lab

The Gietz lab's Definitive Yeast Transformation page

Transposable elements page from Dan Voytas' lab

Dan Gottschling's lab

Virginia Zakian's lab

Chromatin structure page

Lab techniques manual at the Stanford DNA Sequence and Technology Center

Composition

Webster's dictionary	Strunk's "Elements of Style"
Roget's thesaurus	Fowler's "King's English"

Questions? Contact us.

Last modified 2 July '03